pJL 89 pJL89载体质粒图谱、序列、价格、抗性、测

作者：王韵壹、李英、熊慧卿

pJL89载体基本信息

质粒名称: pJL 89
出品公司: Addgene
目录编号: 98558
存储实验室: John Lindbo

载体骨架基本信息
载体名称: pCB301
载体出品公司: --
空载体大小: 4500 bp
完整质粒大小: --
载体修饰: 在35S启动子转录起始位点产生StuI位点，在35S终止子上游插入来自三角洲肝炎病毒的SmaI位点和核酶。
载体类型: 植物表达
筛选标记: --
载体抗性: 卡那霉素
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 低拷贝

插入基因信息
插入基因名称: --
物种来源: --
插入大小: --
基因突变: --
GenBank编号 --
启动子: Enh 35S
标签/融合蛋白: --
克隆方法: --
5’克隆位点: --
3’克隆位点: --
5’测序引物 --
3’测序引物 --

文献引用方法
材料和方法部分: pJL 89 was a gift from John Lindbo (Addgene plasmid # 98558 ; :98558 ; RRID:Addgene_98558)
参考文献部分: TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Lindbo JA. Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. 10.1104/pp.107.106377 PubMed 17720752

pJL89载体质粒图谱和多克隆位点信息

pJL 89质粒图谱

pJL89载体简介 pJL89载体序列

LOCUS pJL 89 4707 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.. ACCESSION . VERSION . KEYWORDS pJL 89 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4707) AUTHORS Lindbo JA TITLE TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. JOURNAL Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. PUBMED 17720752 REFERENCE 2 (bases 1 to 4707) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 FEATURES Location/Qualifiers source 1..4707 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(156..1304) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" CDS complement(1603..2397) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 2606..3137 /label=oriV /note="incP origin of replication" rep_origin 2614..3228 /label=oriV /note="origin of replication for the bacterial F plasmid" misc_feature 3321..3345 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" primer_bind 3425..3441 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 3426..3442 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind 4161..4181 /label=35S promoter /note="CaMV 35S promoter, forward primer" promoter 4170..4215 /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" misc_feature 4685..2 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 acctaagaga aaagagcgtt tattagaata atcggatatt taaagggcgt gaaaaggttt 61 atccgttcgt ccatttgtat gtgcatgcca accacaggag atctcagtaa agcgctggct 121 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca 181 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg 241 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt 301 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc 361 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac 421 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc 481 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg 541 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg 601 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg 661 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg 721 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc 781 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg 841 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa 901 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg 961 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct 1021 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa 1081 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc 1141 tcgatcttgg ccgtagcttg ctgggccatc gagccgacgg actggaaggt ttcgcggggc 1201 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg 1261 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg 1321 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc 1381 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg 1441 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc 1501 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg 1561 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag 1621 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag 1681 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc 1741 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc 1801 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg 1861 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatgggg tgtcttcttc 1921 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa 1981 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct 2041 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc 2101 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc 2161 aaggtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc 2221 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt 2281 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac 2341 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta 2401 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag 2461 acgaactcca attcactgtt ccttgcattc taaaacttta aataccagaa aacagctttt 2521 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca 2581 caattatggg tgatgctgca actcgagcgg gccgggaggg ttcgagaagg gggggcaccc 2641 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata 2701 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa 2761 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc 2821 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta 2881 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc 2941 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac 3001 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc 3061 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat 3121 ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc 3181 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt 3241 ctctagtgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg 3301 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga 3361 caacttaata acacattgcg gacgttttta aagtactggg gtggttttgg taccgggccc 3421 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcaggt caacatggtg 3481 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg 3541 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca 3601 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat 3661 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat 3721 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 3781 caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc 3841 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc 3901 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa 3961 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat 4021 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 4081 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta 4141 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca 4201 tttcatttgg agaggcctcc ttttttccta ggatgcagcc cgggtcggca tggcatctcc 4261 acctcctcgc ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag 4321 ggagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa 4381 tcctgttgcc ggtcttgcga ctagagtccg caaaaatcac cagtctctct ctacaaatct 4441 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg 4501 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat 4561 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc 4621 agcccggccg ggggatccac tagcagattg tcgtttcccg ccttcagttt aaactatcag 4681 tgtttgacag gatatattgg cgggtaa //