L4440载体质粒图谱、序列、价格、抗性、测序引物

作者：杨超月

载体基本信息

质粒名称: L4440
出品公司: 爱迪基因
目录编号: 1654
存储实验室: Andrew Fire

载体骨架基本信息
载体名称: --
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: 2790 bp
完整质粒大小: --
载体修饰: --
载体类型: 蠕虫表达,RNAi
筛选标记: --
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: --
物种来源: --
插入大小: --
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: T7p, T7p, lacZN, OriF1>>, OriF1<<
克隆方法: 限制性内切酶
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: L4440 was a gift from Vladislav Verkhusha (爱迪基因 plasmid # 1654)
参考文献部分:
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755

载体质粒图谱和多克隆位点信息

L4440质粒图谱

载体简介载体序列

LOCUS L4440 2790 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS L4440 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2790) AUTHORS Fire A et al TITLE Fire Lab C. Elegans Vector Kit 1999 REFERENCE 2 (bases 1 to 2790) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 FEATURES Location/Qualifiers source 1..2790 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(368..1228) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 991..1010 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1229..1333) /gene="bla" /label=AmpR promoter rep_origin complement(1359..1814) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1496..1517) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1708..1727 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1941..1963 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1955..1972 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1956..1972 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1982..2000 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 2025..2041 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2026..2042 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2112..2128) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2112..2128) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(2186..2204) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2228..2245) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2399..197) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2479..2498) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 61 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 1021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 1081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 1141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 1201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 1261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 1321 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt 1381 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 1441 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 1501 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 1561 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 1621 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 1681 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 1741 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 1801 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 1861 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 1921 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc 1981 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat 2041 cgataagctt gatatcgaat tcctgcagcc cgggggatcc acgcgtcacg tggctagcca 2101 tggaaccggt ggatccacta gttctagagc ggccgccacc gcggtggagc tcgaattcat 2161 cgatgatatc agatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg 2221 ttgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 2581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 2761 acaggattag cagagcgagg tatgtaggcg //