pPTRII pPTR II载体质粒图谱、序列、价格、抗性、测
出品公司:
Takara
载体名称:
pPTRII
质粒类型:
曲霉表达质粒，大肠杆菌-曲霉穿梭质粒，曲霉表达载体
高拷贝/低拷贝:
高拷贝
克隆方法:
限制性内切酶，多克隆位点
启动子:
Lac
载体大小:
10031 bp
5’测序引物:
M13-R: CAGGAAACAGCTATGAC
3’测序引物:
M13-F: TGTAAAACGACGGCCAGT
载体抗性:
氨苄青霉素
克隆菌株:
DH5 alpha
宿主细胞（系）:
曲霉菌
产品目录号:
3622
稳定性:
瞬表达
组成型/诱导型:
组成型
病毒/非病毒:
非病毒
相关载体:
pPTRI
载体说明书:
pPTRII载体说明书
备注:
pPTRII载体是曲霉表达载体，能够在曲霉菌中进行自我复制。pPTRII载体可以用于多种曲霉菌（Aspergillus species），例如A. oryzae, A . niger, A. terreus and A . fumigatus。pPTRI载体在曲霉菌中的筛选抗性是Pyrithiamine，中文名是抗硫胺素, 也叫吡啶硫铵。 本载体在构建质粒时，可以利用蓝白斑筛选进行阳性插入克隆验证。
pPTR II载体简介
pPTR II是一可以在曲霉菌中可以自主复制的表达载体质粒。该载体具有氨苄西林抗性基因 Ampr作为大肠杆菌筛选抗性。
在曲霉中，筛选基因是抗硫胺素（Pyrithiamine） ，抗性基因为ptr A基因。本质粒具有多克隆位点，包含 Hind III，Pst I，Sma I和Kpn I。
在载体lac Z区域，任何DNA插入到这个区域 使用含有IPTG和X-Gal的平板可以很容易地进行蓝白斑筛选阳性克隆载体。
pPTR II载体序列
LOCUS pTR II Vector 10031 bp DNA circular SYN 23-JUL-2021 COMMENT Created by Takara Bio Inc. COMMENT Cat. No. 3622 FEATURES Location/Qualifiers gene complement(2943..4950) /label="ptrA" /note="pyrithiamine resistance gene useful as a dominant selectable marker in Aspergillus" CDS 4968..5828 /label="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 5999..6587 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 6911..6941 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 6949..6965 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6973..6989 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 6985..7311 /label="lacZ-alpha" misc_feature 7002..7058 /label="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(7059..7075) /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin join(7416..10031,1..2633) /label="AMA1" /note="replication origin from Aspergillus nidulans (Aleksenko and Clutterbuck, 1996)" ORIGIN 1 ctgcagattt tgacctagtt aacaaggcac atcatgctat cacttccaac actcttgctg 61 agcgcgttct gccttgcctt tctggttcta cgaagtatcc agtcccgcct gcaacgccgc 121 agaaacgcca aaaggtgggg ttgtcaaccg ccagctagag ctccatcggg attctttggc 181 attccgccgt tcctcggtgg tcgcagataa ctccctaaat tccctgagct aactctaagt 241 cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat ggccgactta gagttacctc 301 ttgaccgact ttttctttcc tcccccttac atttcgttac cacaacacat tcctatatcc 361 aaactccagg tacataacta gtcgaaatct cttttaaatc tagtcaagaa ctagatactt 421 aaccttcata ctgcttagag atagcctttg aagctcttat ttcgctcttt gtctcactct 481 caccttcctc ctcctcctcc aatccttttt gctcgggact agtccaagac tagtcaacga 541 ttagtatgcc gagcattcgc gataacgatc tccgaaagtc cccagagtac tgtcactatc 601 tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta agccgacccg 661 actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg cctgatatcc 721 atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg agtctactgc 781 tatactagtt tctgactagt tattaactag ttgacaggtt ccctctgcca acagaaacat 841 attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat tgcacaatgg 901 acctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag attacttggt 961 gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg cactgttctc 1021 ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt gagtctaact 1081 agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta agggtgtcga 1141 gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta actactttgc 1201 agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta agtacagcca 1261 ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg tacgccgtgc 1321 atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat gagaaaggaa 1381 aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc tctagccact 1441 ctttatgcag atccgccgtg agggtagcaa ggcagctaaa tcagctggtg agaaaggaac 1501 cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt aagctatcca agctagtttg 1561 ggactagatt ctaactagtc tcagcaccgt gtggttcaaa gccatattgc gagttttggc 1621 gctttttctc atcgattgac gaggcaaagg gagcgagtat gcaacctcgt aagtcagact 1681 cagacaaaga ccaactagtt attgaccagt cttctagaag gctctgttgt ggatcttgag 1741 gccctggaga gttcctccaa caatccggag acaagcaagt cctcgtcgga ctagtcacta 1801 actagactct aactagttgc agacatggat aatgcaaaag agacaagcaa tgaagaaagt 1861 ggtaagacat ttctcctttg tggttctgga ctagtctttg actagtcaca gtcttaaaca 1921 aggaaaatga gcatgaaaat gaggaggaaa aggctgctga gcccgaggaa gtgcagggtg 1981 atggcagaca tggtaggtta ataccttgtt agttattgct agtcactgac tagtcaataa 2041 ctagtctctg aacaccttgc aatcactccg tttgcgcagc tgaacagtgg tgaggataat 2101 agtagtaagt tattctagct tcagagttat aggagactag atactaacta gtattagttg 2161 caactaacct ggatctcaga gactttggcc tcaatctaga atctatctag ttgtcaacta 2221 gactgtggta tcattgtctt ttattttcct agtcctggaa ctagcttcta actagtctcc 2281 ctaatatgtg gctgtcttgt tttttttttt tgtttcccta cccggatatc tagtcccctt 2341 ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga gattagtttc 2401 taactagtct ctaggttttc tatccacctt taattgtaat aataaataca agcaacgttt 2461 atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt gtttagttta 2521 taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata tttttaacaa 2581 gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa gctggctttc 2641 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 2701 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 2761 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 2821 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga 2881 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 2941 aaatggggtg acgatgagcc gctcttgcat ctttgtttgt attatactgt ctttcttgtt 3001 acacataatt attctagaat gccccaccgt tacatacggg acacagccat ttacatatgc 3061 atgtggatta cgagctaacg agttcattca aatctcagaa ctatcacata atcatcattc 3121 ccctatcgtc aaagaccgta agacaaatcc ggttcatgca ctgaacccat tcgggtagtg 3181 agtcatttac tcagcacact cgcgctgacg ctcgtcgaac accttcaatg cctcctcggc 3241 agccttgaca ccactgagaa ccatggcacc gaaggtaggg cccatgcggt taaagccatc 3301 aatttcagac agctccatac cgccgattat caagccctta gtaacctcgc gggtgttctt 3361 gacgatggca tcctcggccg agctcatgtc gagaccacgc atgccaccta gcttgtcgac 3421 gctgcccatg gacaccaagc gcttcgcaca gaaggcgccg aatggcccat cgtgaccagt 3481 ggtactgatg atgacaggag cgttgatagt gttggggtcc atgcaggagt gatcatcgtg 3541 gtgaagggtg accagcgtcc agttgacgac aacaccagca atctgggggt tgccgttctc 3601 ggtcggacgg gtgatcaagt cctcaacagc ggtagcattg aagagcttga cattggggaa 3661 ggagagaacc ttcgacatga gtgtcgaggt aaacagggag gcgtgcttga cgacaacgta 3721 gttggggttt gcgtcctctt cgtaaggaac acccagctcg ttcaggaaga cttccgcggg 3781 acggcgcatg accatagcag aaaagagttg gccacccaac caggcaccgc cacctattgc 3841 acgttagttc cggaaagctg agtgcaaggc aatccatcat ggactactga ccaggagaga 3901 cgctggcctc gacgatagca atcttcaggt ccggacgagc cttggccaag acgtacgcag 3961 tgctcagacc gcaggaacca gcaccaacaa tgacaacgtc actttcagcg tacttgtcca 4021 ggtcctcaaa gtaacgtctg gtcatggcac gagagacctg gctttcgcgg ataggggcga 4081 acttgaactc gtcccacttg ccaccgaaat ggtccaacag cttggtctga gaagctccct 4141 caacggggac ggtctcagaa accacgacct tacccttgag gccggtagcg gccacagtgg 4201 gttcgtagat ggcagctgga ggagacatgt ttcaagttgc aatgactatc atctgttagc 4261 cattccatca acaggaagaa cgagagaagg catgaccctt ttcgctggta ttatccagat 4321 caagttttag ccgtataatc tcagaacgaa cccagtccat cgatgccatg tccttctaga 4381 ctaggatcct agagtctagg gcccagctta gggagggcat gtgaatgcat cgatgactgg 4441 gaacgaacac cggcccacgc caaagacgtt acctaagata ccttgatcat tgtgagagtc 4501 cagccaaaag tattccatga cttccatcgt atgccctcta gagggctaat cgaggagtgt 4561 atttacattg tcggttggtt tgggaactat agaagatggt cagttattcc aatcaccaaa 4621 ggtttatcga agggaggaag acttgttcag tttcgtccga ggacttttgg aattcaaatc 4681 tgagatagag aattgtgtgg gatgagagga aaaggaagaa gatggtgggg ttcagaagga 4741 ggggttgagt taaatagcat gggttgagtc aacgtgataa gggcactata ccgtatagat 4801 cagcggcacc cgattctatc cgttcctttt gctcctcttt agctttgacc ggtgagccgg 4861 acaagaaaca agtgaaatca tcctgacatc ggcggacgat ctcctagctt ttacatttcg 4921 ttaccaatgg gatcccgtaa tcaattgccc attgaaaaag gaagagtatg agtattcaac 4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 6001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 6061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 6121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 6181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 6721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 6781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 6841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 6901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 6961 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg 7021 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt 7081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 7141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 7201 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 7261 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 7321 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt 7381 tcgctttctt cccttccttt ctcgccacgt tcgccagctt attttttgta tactgttttg 7441 tgatagcacg aagtttttcc acggtatctt gttaaaaata tatatttgtg gcgggcttac 7501 ctacatcaaa ttaataagag actaattata aactaaacac acaagcaagc tactttaggg 7561 taaaagttta taaatgcttt tgacgtataa acgttgcttg tatttattat tacaattaaa 7621 ggtggataga aaacctagag actagttaga aactaatctc aggtttgcgt taaactaaat 7681 cagagcccga gaggttaaca gaacctagaa ggggactaga tatccgggta gggaaacaaa 7741 aaaaaaaaac aagacagcca catattaggg agactagtta gaagctagtt ccaggactag 7801 gaaaataaaa gacaatgata ccacagtcta gttgacaact agatagattc tagattgagg 7861 ccaaagtctc tgagatccag gttagttgca actaatacta gttagtatct agtctcctat 7921 aactctgaag ctagaataac ttactactat tatcctcacc actgttcagc tgcgcaaacg 7981 gagtgattgc aaggtgttca gagactagtt attgactagt cagtgactag caataactaa 8041 caaggtatta acctaccatg tctgccatca ccctgcactt cctcgggctc agcagccttt 8101 tcctcctcat tttcatgctc attttccttg tttaagactg tgactagtca aagactagtc 8161 cagaaccaca aaggagaaat gtcttaccac tttcttcatt gcttgtctct tttgcattat 8221 ccatgtctgc aactagttag agtctagtta gtgactagtc cgacgaggac ttgcttgtct 8281 ccggattgtt ggaggaactc tccagggcct caagatccac aacagagcct tctagaagac 8341 tggtcaataa ctagttggtc tttgtctgag tctgacttac gaggttgcat actcgctccc 8401 tttgcctcgt caatcgatga gaaaaagcgc caaaactcgc aatatggctt tgaaccacac 8461 ggtgctgaga ctagttagaa tctagtccca aactagcttg gatagcttac ctttgccctt 8521 tgcgttgcga caggtcttgc agggtatggt tcctttctca ccagctgatt tagctgcctt 8581 gctaccctca cggcggatct gcataaagag tggctagagg ttataaatta gcactgatcc 8641 taggtacggg gctgaatgta acttgccttt cctttctcat cgcgcggcaa gacaggcttg 8701 ctcaaattcc taccagtcac aggggtatgc acggcgtacg gaccacttga actagtcaca 8761 gattagttag caactagtct gcattgaatg gctgtactta cgggccctcg ccattgtcct 8821 gatcatttcc agcttcaccc tcgttgctgc aaagtagtta gtgactagtc aaggactagt 8881 tgaaatggga gaagaaactc acgaattctc gacaccctta gtattgtggt ccttggactt 8941 ggtgctgcta tatattagct aatacactag ttagactcac agaaacttac gcagctcgct 9001 tgcgcttctt ggtaggagtc ggggttggga gaacagtgcc ttcaaacaag ccttcatacc 9061 atgctacttg actagtcagg gactagtcac caagtaatct agataggact tgcctttggc 9121 ctccatcagt tccttcatag tgggaggtcc attgtgcaat gtaaactcca tgccgtggga 9181 gttcttgtcc ttcaagtgct tgaccaatat gtttctgttg gcagagggaa cctgtcaact 9241 agttaataac tagtcagaaa ctagtatagc agtagactca ctgtacgctt gaggcatccc 9301 ttcactcggc agtagacttc atatggatgg atatcaggca cgccattgtc gtcctgtgga 9361 ctagtcagta actaggctta aagctagtcg ggtcggctta ctatcttgaa atccggcagc 9421 gtaagctccc cgtccttaac tgcctcgaga tagtgacagt actctgggga ctttcggaga 9481 tcgttatcgc gaatgctcgg catactaatc gttgactagt cttggactag tcccgagcaa 9541 aaaggattgg aggaggagga ggaaggtgag agtgagacaa agagcgaaat aagagcttca 9601 aaggctatct ctaagcagta tgaaggttaa gtatctagtt cttgactaga tttaaaagag 9661 atttcgacta gttatgtacc tggagtttgg atataggaat gtgttgtggt aacgaaatgt 9721 aagggggagg aaagaaaaag tcggtcaaga ggtaactcta agtcggccat tcctttttgg 9781 gaggcgctaa ccataaacgg catggtcgac ttagagttag ctcagggaat ttagggagtt 9841 atctgcgacc actggactct caggctttcc attggggtac taacatagcc atcaaatgcc 9901 ggcccgtgtt taatctcctc gagatggctg ggctcacatg cgttctactc aaaaactctt 9961 atacctaggc cctctcccaa tcgtgtatgt ttggaccttc ctctctcaca ctttggataa 10021 catcaagcat a //
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