pSTOP1622载体质粒图谱、序列、价格、抗性、测序

作者：张馨予

载体基本信息

出品公司: MoBiTec
载体名称: pSTOP1622
质粒类型: 枯草芽孢杆菌表达载体, 枯草杆菌表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶，多克隆位点
启动子: PxylA
载体大小: 6.5 kb
5’测序引物: --
3’测序引物: pBR322ori-F: GGGAAACGCCTGGTATCTTT
载体抗性: 氨苄青霉素
筛选标记: 四环素（枯草芽孢杆菌）
克隆菌株: DH5 alpha
产品目录号: #BMEG25
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒
载体说明书: pSTOP1622载体说明书

载体质粒图谱和多克隆位点信息

pSTOP1622载体图谱

pSTOP1622多克隆位点

载体简介

pSTOP1622质粒是一种枯草芽孢杆菌载体。枯草芽孢杆菌是一种杆状、非致病性革兰氏阳性细菌，能够在多种碳源上生长。与其他土壤细菌一样，它能够向培养基中分泌大量蛋白质（高达0.5 g/L重组蛋白）。这种特性，再加上它在许多生态环境中生长的能力，如肉类工业的废物、糖渣或石化废水，使其成为工业规模生产重组蛋白的有趣生物体。除枯草杆菌外，它还被用作革兰氏阳性模式生物，用于研究生物化学、孢子形成和噬菌体等几个研究课题。

载体序列

LOCUS pSTOP1622 6535 bp ds-DNA circular SYN 26-NOV-2017 DEFINITION synthetic circular DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6535) FEATURES Location/Qualifiers source 1..6535 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 398..432 /label=xylA Promoter misc_feature 500..512 /label=RBS misc_feature 520..608 /label=MCS rep_origin complement(741..1329) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1500..2360) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2361..2465) /gene="bla" /label=AmpR promoter CDS complement(2629..4005) /codon_start=1 /gene="Bacillus subtilis tetL" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF " CDS complement(4205..5209) /codon_start=1 /gene="repB" /product="RepB replication protein" /label=repB /note="from Enterococcus faecalis plasmid pAM-alpha-1" /db_xref="GI:22652809" /protein_id="AAN03827.1" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" CDS complement(5663..252) /codon_start=1 /label=xylR /translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS" ORIGIN 1 aattccgtta agatagtcga ctcctatatc aataccaatc gagtagcctg cattcttatt 61 aaaaacaagc attacaggtc ttctgccgcc tctagattgc cctgccccaa tttcaaaaat 121 aaaatctttt tcaagcagtg tatttacttg agaggagaca gtagacttgt ttaatcctgt 181 aatctcagag agagttgccc tggagacagg ggagttcttc aaaatttcat ctaatattaa 241 tttttgattc atttttttta ctaaagcttg atctgcaatt tgaataataa ccactccttt 301 gtttatccac cgaactaagt tggtgttttt tgaagcttga attagatatt taaaagtatc 361 atatctaata ttataactaa attttctaaa aaaaacattg aaataaacat ttattttgta 421 tatgatgaga taaagttagt ttattggata aacaaactaa ctcaattaag atagttgatg 481 gataaacttg ttcacttaaa tcaaaggggg aaatgtacaa tggtccaaac tagttcgaag 541 atctccggag ctcccgggat ccggtaccgg ccgcatgccg gctaaacctc gcgaacggat 601 tcaccggtcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca 661 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct 721 cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 1021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 1081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 1141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 1201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 1261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 1321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 1381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 1441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 1501 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 1561 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 1621 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 1681 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 1741 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 1801 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 1861 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 1921 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 1981 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 2041 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 2101 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 2161 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 2221 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 2281 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 2341 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 2401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 2461 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 2521 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cctgttataa 2581 aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgattt agaaatccct 2641 ttgagaatgt ttatatacat tcaaggtaac cagccaacta atgacaatga ttcctgaaaa 2701 aagtaataac aaattactat acagataagt tgactgatca acttccatag gtaacaacct 2761 ttgatcaagt aagggtatgg ataataaacc acctacaatt gcaatacctg ttccctctga 2821 taaaaagctg gtaaagttaa gcaaactcat tccagcacca gcttcctgct gtttcaagct 2881 acttgaaaca attgttgata taactgtttt ggtgaacgaa agcccaccta aaacaaatac 2941 gattataatt gtcatgaacc atgatgttgt ttctaaaaga aaggaagcag ttaaaaagct 3001 aacagaaaga aatgtaactc cgatgtttaa cacgtataaa ggacctcttc tatcaacaag 3061 tatcccacca atgtagccga aaataatgac actcattgtt ccagggaaaa taattacact 3121 tccgatttcg gcagtactta gctggtgaac atctttcatc atataaggaa ccatagagac 3181 aaaccctgct actgttccaa atataattcc cccacaaaga actccaatca taaaaggtat 3241 atttttccct aatccgggat caacaaaagg atctgttact ttcctgatat gttttacaaa 3301 tatcaggaat gacagcacgc taacgataag aaaagaaatg ctatatgatg ttgtaaacaa 3361 cataaaaaat acaatgccta cagacattag tataattcct ttgatatcaa aatgaccttt 3421 tatccttact tctttcttta ataatttcat aagaaacgga acagtgataa ttgttatcat 3481 aggaatgagt agaagatagg accaatgaat ataatgggct atcattccac caatcgctgg 3541 accgactcct tctcccatgg ctactatcga tccaataaga ccaaatgctt tacccctatt 3601 ttcctttgga atatagcgcg caactacaac cattacgagt gctggaaatg cagctgcacc 3661 agccccttga ataaaacgag ccataataag taaggaaaag aaagaatggc caacaaaccc 3721 aattaccgac ccgaaacaat ttattataat tccaaatagg agtaaccttt tgatgcctaa 3781 ttgatcagat agctttccat atacagctgt tccaatggaa aaggttaaca taaaggctgt 3841 gttcacccag tttgtactcg caggtggttt attaaaatca tttgcaatat caggtaatga 3901 gacgttcaaa accatttcat ttaatacgct aaaaaaagat aaaatgcaaa gccaaattaa 3961 aatttggttg tgtcgtaaat tcgattgtga ataggatgta ttcacatttc accctccaat 4021 aatgagggca gacgtagttt atagggttaa tgatacgctt ccctctttta attgaaccct 4081 gttacattca ttacacttca taattaattc ctcctaaact tgattaaaac attttaccac 4141 atataaacta agttttaaat tcagtatttc atcacttata caacaatatg gcccgtttgt 4201 tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa 4261 atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt 4321 catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt 4381 tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg 4441 tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat 4501 ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta 4561 atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat 4621 tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta taagaattat 4681 ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt 4741 ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt 4801 cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct 4861 tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc 4921 tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt 4981 cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt actctttcag 5041 cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt 5101 ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat 5161 ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta 5221 tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg 5281 ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa 5341 taaaaatgac gttatttcta tatgtatcaa gataagaaag aacaagttca aaaccatcaa 5401 aaaaagacac cttttcaggt gcttttttta ttttataaac tcattccctg atctcgactt 5461 cgttcttttt ttacctctcg gttatgagtt agttcaaatt cgttcttttt aggttctaaa 5521 tcgtgttttt cttggaattg tgctgtttta tcctttacct tgtctacaaa ccccttaaaa 5581 acgtttttaa aggcttttaa gccgtctgta cgttccttaa gatcaacgtg atataggttt 5641 gctaaccttt gcgttcactt aactaactta taggggtaac acttaaaaaa gaatcaataa 5701 cgatagaaac cgctcctaaa gcaggtgcat tttttcctaa cgaagaaggc aatagttcac 5761 atttattgtc taaatgagaa tggactctag aagaaacttc gtttttaatc gtatttaaaa 5821 caatgggatg agattcaatt atatgatttc tcaagataac agcttctata tcaaatgtat 5881 taaggatatt ggttaatcca attccgatat aaaagccaaa gttttgaagt gcatttaaca 5941 tttctacatc atttttattt gcgcgttcca caatctcttt tcgagaaata ttcttttctt 6001 ctttagagag cgaagccagt aacgcttttt cagaagcata taattcccaa cagcctcgat 6061 ttccacagct gcatttgggt ccattaaaat ctatcgtcat atgacccatt tccccagaaa 6121 aaccctgaac acctttatac aattcgttgt taataacaag tccagttcca attccgatat 6181 taatactgat gtaaacgatg ttttcatagt tttttgtcat accaaatact ttttcaccgt 6241 atgctcctgc attagcttca ttttcaacaa aaaccggaac attaaactca ctctcaatta 6301 aaaactgcaa atctttgata ttccaattta agttaggcat gaaaataatt tgctgatgac 6361 gatctacaag gcctggaaca caaattccta ttccgactag accataaggg gactcaggca 6421 tatgggttac aaaaccatga ataagtgcaa ataaaatctc ttttacttca ctagcggaag 6481 aactagacaa gtcagaagtc ttctcgagaa taatatttcc ttctaagtcg gttag //