ZsGreen载体质粒图谱、序列、说明书、价格
RNAi-Ready pSIREN-RetroQ-ZsGreen载体质粒图谱、序列、说明书、价格
2022-04-02 08:45 来源: HonorGene生物资源库
原标题:RNAi-Ready pSIREN-RetroQ-ZsGreen载体质粒图谱、序列、说明书、价格
产品具体信息请参见:
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质粒类型:RNAi载体
启动子:Human U6
载体大小:6568 bp
5' 测序引物及序列:U6:5'-ATGGACTATCATATGCTTACCGTA-3'
载体抗性:Ampicillin (氨苄青霉素)
价格:询价
载体描述:
RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.
请访问以下页面下载【RNAi-Ready pSIREN-RetroQ-ZsGreen】产品说明书及载体序列:
质粒图谱:
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