荧光与化学发光成像仪
【Cool Imager™】- 高灵敏生物发光与荧光数字成像仪 (IMGR002, IMGR003)
一、产品背景常规的生物图像与生物信号用肉眼观察灵敏度低或根本无法进行肉眼
观察。常常需要采用酶显色或放射性同位素放大后进行观察。近年兴起的Chemiluminescence(化学发光)因其极高灵敏度与优越的线性关系得到普遍应用。用光学照相机照像或胶片显影法显示结果则需要暗室以及胶片,照相纸,冲洗液等耗材。图像的定量分析与后期处理也较困难。相比之下,通过数字相机的数字成像技术则有许多优点。国内市场上出售的电泳图像成像系统因灵敏度低而不能用于显示化学自发光反应 (Chemiluminescence)以及荧光示踪。结合现代生物学,光学,半导体及计算机技术,我公司开发了高灵敏度的数字成像系统(Cool Imager)。 Cool Imager为多用途数字成像及图像分析工作站,主要用于酶催化的化 学自发光反应与荧光图谱。由于对摄像系统采用了深低温冷却(-45oC)技术,灵敏度较传统的酶底物显色法提高约2000倍。完全成像系统包括所有硬件、并已经安装系统与分析软件,开箱后即可使用。高度集成的系统已经包括微机,不需要另配计算机。系统已经优化。系统启动与关闭迅速。采像单元可每分钟采积并累加近2000幅图。最高累加图幅达200,000幅图,采像时间可达25分钟。一般取图5,000幅左右即可在计算机屏幕清晰显示蛋白印迹图或分子杂交图。由于采图数是任意可变的,Cool Imager可扩展用于摄取DNA/RNA凝胶图,考马氏亮兰及银染的蛋白凝胶图,以及蛋白荧光图谱。
本工作台可连接显微摄像头摄取彩色显微镜图像。
二、技术指标
系统冷却达-45oC,可以使微弱的发光成像。曝光25分钟、累集10,000幅图没有明显杂色背景。
三、技术参数(CoolImager II)
MAIN UNIT Complete System Cool Imager™ is an all-in-one unit and
comes with all the hardware including
the monitor, keyboard, mouse,
pre-installed operating system and
analytical software. After you receive
the units, plug in and use; that is it.
No custom hardware and software
installation are required. High Integration The unit is integrated with the
computer-board with an embedded CPU, the
control board and operating panel,
optical devises, a frame grabber,
lights, filters and a mini darkroom
chamber. This specific design increases
efficiency and stability of the
workstation. Low-noise Technology
Low-noise technology is applied to all
the electronic components in the unit to
prevent the interruption of
electro-noise and reduce vibration,
which makes the design of highly
integrated system possible and enhances
the stability of the unit. Sensitivity Detects as little as ~0.5 fg (10-17g)
of HRP with Lightgen™ substrates and
completely eliminates the use of X-ray
film for chemiluminescent imaging.
Imaging Capacity Cool imager can integrate ~30 frames per
second and collect ~50,000 frames in
~30min, and its sensitivity is better
than autoradiography using radioisotope
p32
or S35. Darkroom The mini darkroom is light-proofed by a
design with multi-staged, magnetic
sealing strips, which permit the door to
open and close easily. Self-contained Unit There is no external camera or lens
outside of the unit.
All electronic and optical components
are enclosed inside the unit, which
provides a moisture-proof, dust-proof
environment and extend the service life
of the electronic and optical
components. Power Operation Workstation uses a flat control panel to
turn on and off the operation program
and also controls image capturing, light
source, power lens and camera functions. SOFT-Ware Operation
Pre-installed Windows®
(Microsoft) or Linux®
(Redhat) operation program Imaging & Analyzing Software
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