磷脂脂肪酸法在土壤微生物群落分析中的应用

作者：王韵壹、李英、熊慧卿

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姚晓东, 王娓, 曾辉. 磷脂脂肪酸法在土壤微生物群落分析中的应用[J]. 微生物学通报, 2016, 43(9): 2086-2095.

YAO Xiao-dong, WANG Wei, ZENG Hui. Application of phospholipid fatty acid method in analyzing soil microbial community composition[J]. Microbiology China, 2016, 43(9): 2086-2095.

磷脂脂肪酸法在土壤微生物群落分析中的应用

姚晓东1,2, 王娓2

磷脂脂肪酸法在土壤微生物群落分析中的应用

, 曾辉1,2

1. 北京大学深圳研究生院广东深圳 518055 ;
2. 北京大学城市与环境学院北京 100871

收稿日期: 2016-01-07; 接受日期: 2016-04-20; 优先数字出版日期(): 2016-05-11

基金项目: 国家自然科学基金项目(No.31222011，31270363，31021001，31321061);国家重点基础研究发展规划项目(973 计划)(No. 2013CB956303)

*通讯作者: Tel/Fax：86-10-62755923. E-mail:wangw@urban.pku.edu.cn

摘要：土壤微生物群落的组成一直是土壤学、微生物学和生态学研究的热点问题。我国在这方面的研究处于国际前列，越来越多的研究成果在国际重要刊物上发表。而磷脂脂肪酸(PLFA)法在土壤微生物群落分析中占有举足轻重的地位，国内外学者都热衷于使用该方法。但是PLFA法的使用仍存在一些不足的地方，需要研究学者们慎重使用。本文综述了国际上相关研究，概述了PLFA方法使用的发展历史，应用及挑战。总结了使用和数据解读时需要注意的问题，整理了PLFA法相关的生物标记以及与新方法结合的设想，方便以后研究的开展。

关键词：磷脂脂肪酸土壤微生物生物表征

Application of phospholipid fatty acid method in analyzing soil microbial community composition

YAO Xiao-dong1,2, WANG Wei2

磷脂脂肪酸法在土壤微生物群落分析中的应用

, ZENG Hui1,2

1. Shenzhen Graduate School，Peking University，Shenzhen，Guangdong 518055，China ;
2. College of Urban and Environment，Peking University，Beijing 100871，China

Received: January 07, 2016; Accepted: April 20, 2016; Published online(): May 11, 2016

Foundation item: National Natural Science Foundation of China(No.31222011，31270363，31021001，31321061);National Basic Research Program of China(973 Program)(No.2013CB956303)

*Corresponding author: Tel/Fax：86-10-62755923. E-mail:wangw@urban.pku.edu.cn.

Abstract: Soil microbial community composition was the hot issue of soil science，microbiology and ecology. This research conducted in China was in the international forefront and more and more relevant research has been published in high influential international journals. The phospholipid fatty acid(PLFA)method plays a significant role in the analysis of soil microbial communities，both domestic and foreign. But it also has some shortages，which should arouse the concern of research scholars. This paper reviewed the related research focusing on the development history and the application of PLFA method. This review highlights the caution when using and interpreting PLFA data，summarizes the PLFA biomarker and provides some suggestions towards the combination with the new approach in order to facilitate future studies.