果蝇遗传学实验
This course is a discovery-based research class which will offer basic training in biological research including techniques in genetics, model organisms, bioinformatics, genomics and fluorescent microscopy. In addition to conducting original research in Drosophila genetics, students will attend lectures that discuss fundamental concepts in genetics and developmental biology, how to use online databases for genome information and research literature, important considerations in laboratory ethics, and scientific record keeping.
Course Description
One hour of weekly lecture and 3 hours of scheduled lab time. The lab will be open additional hours for unscheduled laboratory work.
This course is a discovery-based research class which will offer basic training in biological research, including techniques in genetics, model organisms, bioinformatics, genomics and microscopy. In addition to conducting original research in Drosophila genetics, students will attend lectures that discuss fundamental concepts in genetics and developmental biology, how to use online databases for genome information and research literature, important considerations in laboratory ethics, and scientific record keeping.
Assignment and Grading:
Midterm and Final exams are open-book and take-home. In addition your grade will be based on lab effort, classroom participation, computer exercises, quizzes, your lab notebook, and writing assignments.
Quizzes: They are used to test your understandings of lecture and lab instruction materials. They will be taken during the beginning of lab sections.
Lab notebook: You will be given a bound notebook for recording your experimental data. It will be checked for the completeness and neatness of your record.
Lab effort: Your effort will be reflected on how well you maintain the fly stocks, keep track of your crosses, and generate quality data. The instructor will determine how well you perform in the research at the end of the semester. In addition, your results will be reflected in the lab notebook and final report. Participation during class and lab discussions is a very important component of this class.
Computer exercises: There is usually one computer exercise work sheet to be completed for each computer assignment.
Midterm: The midterm will be a written scientific research proposal based upon your current project.
Final: The Final paper will summarize your work in the class. The paper will be modeled after original research articles published in the journal “Cell”.
The final grade for the course will be calculated as below:
Lab component
Notebook
100
Lab effort & Participation
250
Computer exercises
150
Quizzes(2)
100
Midterm and Final Paper
Midterm report
150
Final Paper
250
Total (100%)
1000 points
Tentative schedule subject to change
Week 1 Importance of research in genetics
Week 2 Classical genetics and scientific record keeping
Week 3 Genetic crosses; virtual flylab
Week 4 Recombination mapping
Quiz 1 during lab section
Week 5 Mutational analysis
Week 6 PubMed and beyond- literature research
Week 7 Gal4/UAS system
Quiz 2 during lab section
Week 8 Differential gene expression
Midterm report due
Week 9 Drosophila Development I
Week 10 Drosophila Development II
Week 11 Flybase
Week 12 Conducting BLAST searches
Week 13 Manuscript writing basics
Week 14 Cell communication and signal transduction
Week 15 Research ethics
Week 16 How to think like a geneticist
Final Paper Due
Instructor: Dr. John Olson e-mail: jmolson@pku.edu.cn
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