不同性别人群免疫球蛋白G
孙扬, 王友信, 张杰, 于鑫玮, 盖思齐, 王皓, 董晶, 郭秀花, 王嵬 . 不同性别人群免疫球蛋白G N-糖基化结构谱型比较[J]. 中华流行病学杂志, 2016, 37(10): 1409-1412
Sun Yang, Wang Youxin, Zhang Jie, Yu Xinwei, Ge Siqi, Wang Hao, Dong Jing, Guo Xiuhua, Wang Wei . Comparison of gender specific structure profiles of immunoglobulin G N-glycans[J]. CHINESE JOURNAL OF EPIDEMIOLOGY, 2016, 37(10): 1409-1412.
不同性别人群免疫球蛋白G N-糖基化结构谱型比较
孙扬1, 王友信1, 张杰1, 于鑫玮1, 盖思齐1, 王皓1, 董晶2, 郭秀花1
1.
100069 北京, 首都医科大学临床流行病学北京市重点实验室, 公共卫生学院;
2.
100050 北京, 首都医科大学宣武医院体检中心;
3.
WA6027 澳大利亚珀斯, 埃迪斯科文大学医学科学院
收稿日期: 2016-06-12
项目资助: 国家自然科学基金（81530087，81373099）
通讯作者: 郭秀花,Email:guoxiuh@ccmu.edu.cn 王嵬,Email:wei.wang@ecu.edu.un
摘要: 目的 对不同性别人群的免疫球蛋白G（IgG） N-糖基结构谱型进行分析比较。 方法 采用整群抽样，以2012年1-6月在北京市宣武医院体检的669名居民为研究对象，留取空腹静脉血5 ml，对IgG N-糖基进行分离、纯化、标记后，采用超高压液相色谱串联质谱法对IgG N-糖基进行检测，最终得到24个色谱峰，每个峰代表不同的糖基结构。不同性别人群的糖基结构比较采用两独立样本t检验或非参数检验，P<0.05为差异有统计学意义。 结果 669名参与者中男性235人（35.13%），女性434人（64.87%），平均年龄为（44.23±9.82）岁。女性核心岩藻糖基化水平Fn（Z=-2.192，P=0.028）高于男性；女性半乳糖基化水平G0n（Z=-7.898，P<0.001）、G1n（Z=-2.343，P=0.019）低于男性，而G2n（Z=-8.414，P<0.001）水平高于男性；女性唾液酸化水平FtotalS1/FtotalS2（Z=-5.049，P<0.001）、FS1/FS2（Z=-3.336，P=0.001）高于男性。 结论 IgG N-糖基结构水平可能与性别有关。
关键词： IgG N-糖基化 性别
Comparison of gender specific structure profiles of immunoglobulin G N-glycans
Sun Yang1, Wang Youxin1, Zhang Jie1, Yu Xinwei1, Ge Siqi1, Wang Hao1, Dong Jing2, Guo Xiuhua1
1.
Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China;
2.
Center for Physical Examination, Xuanwu Hospital, Capital Medical University, Beijing 100050, China;
3.
School of Medical Sciences and Health, Edith Cowan University, Joondalup WA6027, Australia
Abstract: Objective To analyze and compare the gender specific structure profiles of immunoglobulin G(IgG) N-glycans and provide evidence to understand the distribution of the structure of IgG N-glycans in general population. Methods A cluster sampling was conducted to randomly select 669 participants from Beijing Xuanwu Hospital during January-June 2012. Venous blood sample (5 ml) was collected from each participant with vacuum negative pressure tube containing EDTA. Plasma IgG N-glycome was determined using ultra-performance liquid chromatography (UPLC) on Waters BEH Glycan chromatography column and mass spectrometry after the separation, purification and labeling. Finally, we got 24 chromatographic peaks. Each peak represented a different carbohydrate structure. Independent sample t-test or nonparametric test were used to compare the gender specific difference, P<0.05 was regarded as significant. Results A total of 669 healthy participants were included, including 235 men (35.13%) and 434 women (64.87%). The average age of the participants was (44.23±9.82) years. The core fucosylation level and the galactosylation level of females were higher than those of males[Fn(Z=-2.192, P=0.028), G0n(Z=-7.898, P<0.001), G1n(Z=-2.343, P=0.019), G2n(Z=-8.414, P<0.001)], but FtotalS1/FtotalS2(Z=-5.049, P<0.001) and FS1/FS2 (Z=-3.336, P=0.001) of females were higher than those of males, indicating a higher saliva acidification level in males than in females. Conclusion IgG N-glycosylation levels might be gender dependent.
Key words: IgG N-glycosylation Gender
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