新生大鼠心肌细胞原代培养方法的改良

作者：杨超月

新生大鼠心肌细胞原代培养方法的改良被引量： Improvement of primary culture of cardiac myocytes in neonatal rat

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摘要目的：探讨更为简便的新生大鼠心肌细胞原代培养的方法，使分离的心肌细胞达到理想的数量、存活率和纯度。方法：用胰蛋白酶和Ⅱ型胶原酶联合消化，分离新生大鼠心肌细胞，进行体外培养，观察心肌细胞形态学变化，并以细胞免疫荧光进行心肌细胞纯度鉴定。结果：心肌细胞分离良好，收获细胞数量及存活率高，可贴壁搏动生长，免疫荧光显示培养的心肌细胞纯度达95％以上。结论：改良的细胞培养技术可获得生长状态良好的心肌细胞。 Objective ：To discuss how the more convenient primary culture method of rats＇cardiac myocytes to increase the quantity, livability and purity of isolated cells. Methods： Neonatal rats＇ cardiac myocytes were isolated by co-digestion of trypsin and collagenase II,and then cultured in vitro.Morphocytology changes were observed and cell purity was evaluated by immunofluorescence. Results： Isolation of cardiomyocytes was with a good quantity and high livability.Cells adhered and impulsed well. Immunofluorescence staining confirmed the cell with purity higher than 95%. Conclusion： The modified cell culture method can obtain the Cardiomyocytes with good growth state.

作者王佳南张晓刚汤为学郑丽娜 WANG Jia-nan,et al （Department of Cardiology,the First Affiliated Hospital, Chongqing Medical University ）

机构地区重庆医科大学附属第一医院心内科重庆医科大学病理生理学教研室

出处《重庆医科大学学报》 CAS CSCD 北大核心 2009年第5期 600-603,共4页 Journal of Chongqing Medical University

关键词心肌细胞原代培养新生大鼠 Cardiac myocyte Primary Culture Neonatal rat

分类号 Q813.11 [生物学—生物工程]

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