自发光镧系金属有机框架作为电化学发光免疫分

作者：杨超月

本期文章：《美国化学会志》：Online/在线发表

济南大学魏琴团队报道了一种新的自发光镧系金属有机框架作为电化学发光免疫分析的信号探针。相关研究成果发表在2020年12月28日出版的国际学术期刊《美国化学会志》。

电化学发光（ECL）在免疫分析临床诊断中的成功应用要求开发新的ECL信号探针。

该文中，研究人员报告了镧系金属-有机框架（LMOFs）作为ECL免疫分析中的ECL信号发射器。以含Eu（III）离子和5-硼邻苯二甲酸（5-bop）的前驱体为原料，制备了可用于调节光学性能的LMOFs。对ECL发光机理的研究表明，紫外光子激发5-bop产生三重态，激发Eu（III）离子产生红光发射。缺少电子的硼酸降低了5-bop三重态到Eu（III）离子的能量转移效率，因此，二者在单次激发时都被高效激发。此外，通过逐步调整Ni/Fe的原子比，制备了活性中心更多、稳定性更好、导电性更好的NiFe复合材料（Ni/Fe1:1）。

结果表明，以细胞角蛋白21-1（cyfra21-1）为靶检测模型，自发光铕LMOFs在ECL免疫分析中表现出良好的性能，最低检测限为0.126 pg mL-1。采用LMOFs作为信号探针，大大降低了假阳性/假阴性的概率。

该策略为镧系金属在分析化学中的应用，特别是在其他疾病标志物的检测中提供了更多的可能性。

附：英文原文

Title: Self-Luminescent Lanthanide Metal–Organic Frameworks as Signal Probes in Electrochemiluminescence Immunoassay

Author: Yaoguang Wang, Guanhui Zhao, Hong Chi, Shenghong Yang, Qingfen Niu, Dan Wu, Wei Cao, Tianduo Li, Hongmin Ma, Qin Wei

Issue&Volume: December 28, 2020

Abstract: The successful use of electrochemiluminescence (ECL) in immunoassay for clinical diagnosis requires development of novel ECL signal probes. Herein, we report lanthanide (Ln) metal–organic frameworks (LMOFs) as ECL signal emitters in the ECL immunoassay. The LMOFs were prepared from precursors containing Eu (III) ions and 5-boronoisophthalic acid (5-bop), which could be utilized to adjust optical properties. Investigations of ECL emission mechanisms revealed that 5-bop was excited with ultraviolet photons to generate a triplet-state, which then triggered Eu (III) ions for red emission. The electron-deficient boric acid decreased the energy-transfer efficiency from the triplet-state of 5-bop to Eu (III) ions; consequently, both were excited with high-efficiency at single excitation. In addition, by progressively tailoring the atomic ratios of Ni/Fe, NiFe composites (Ni/Fe 1:1) were synthesized with more available active sites, enhanced stability, and excellent conductivity. As a result, the self-luminescent europium LMOFs displayed excellent performance characteristics in an ECL immunoassay with a minimum detectable limit of 0.126 pg mL–1, using Cytokeratins21-1 (cyfra21-1) as the target detection model. The probability of false positive/false negative was reduced dramatically by using LMOFs as signal probes. This proposed strategy provides more possibilities for the application of lanthanide metals in analytical chemistry, especially in the detection of other disease markers.

DOI: 10.1021/jacs.0c12449

Source: https://pubs.acs.org/doi/10.1021/jacs.0c12449

期刊信息

JACS：《美国化学会志》，创刊于1879年。隶属于美国化学会，最新IF：14.612
官方网址：https://pubs.acs.org/journal/jacsat
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