温控电喷雾质谱对近端DNA结构域相互作用的新认

作者：王韵壹、李英、熊慧卿

本期文章：《德国应用化学》：Online/在线发表

瑞士苏黎世联邦理工学院Renato Zenobi团队基于温控电喷雾质谱对近端DNA结构域相互作用进行了研究。相关研究成果发表在2021年4月6日出版的国际学术期刊《德国应用化学》。

四链体是许多细胞过程所必需的非标准核酸结构。由多个结构域组成的杂交四重-双链寡核苷酸组装体由于其结构的复杂性，对传统生物物理方法的研究具有挑战性。

该文中，研究人员介绍了一种基于结合定制的温控纳米电喷雾电离（TCnESI）源的天然质谱（MS）新方法，旨在研究近端DNA结构域之间的相互作用。热变性实验旨在观察从生物相关结构衍生的多链寡核苷酸结构的展开，并鉴定展开的中间产物。

利用TCnESI-MS，研究人员在一个实验中观察到了DNA近端结构域的Tm和热力学特性随结构域数目、位置和顺序的变化。

附：英文原文

Title: Novel Insight into Proximal DNA Domain Interactions from Temperature‐Controlled Electrospray Ionization Mass Spectrometry

Author: Adam Pru&#x, Adrien Marchand, Renato Zenobi

Issue&Volume: 2021-04-06

Abstract: Quadruplexes are non‐canonical nucleic acid structures essential for many cellular processes. Hybrid quadruplex‐duplex oligonucleotide assemblies comprised of multiple domains are challenging to study with conventional biophysical methods due to their structural complexity. Here, we introduce a novel method based on native mass spectrometry (MS) coupled with a custom‐built temperature‐controlled nanoelectrospray ionization (TCnESI) source designed to investigate interactions between proximal DNA domains. Thermal denaturation experiments were planned to observe unfolding of multi‐stranded oligonucleotide constructs derived from biologically relevant structures and to identify unfolding intermediates. Using the TCnESI MS, we observed changes in T m and thermodynamic characteristics of proximal DNA domains depending on the number of domains, their position, and order in a single experiment.

DOI: 10.1002/anie.202016757

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202016757

期刊信息

Angewandte Chemie：《德国应用化学》，创刊于1887年。隶属于德国化学会，最新IF：12.959
官方网址：https://onlinelibrary.wiley.com/journal/15213773
投稿链接：https://www.editorialmanager.com/anie/default.aspx