基因芯片技术检测分枝杆菌和异烟肼、利福平耐

作者：杨超月

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摘要目的探讨基因芯片技术检测分枝杆菌和异烟肼(INH)、利福平(RFP)耐药性在结核性脓胸诊断中的临床应用价值。方法收集2011年1月至2015年12月临床疑似结核性脓胸患者182例的脓液标本,分别应用基因芯片技术和快速培养法进行检测,比较两种方法的特异性和敏感性;同时选取36例临床确诊为结核性脓胸患者,且基因芯片法和MGIT培养法均为阳性的标本进行INH、RFP耐药性检测。以MGIT培养法的药敏结果为标准,评价基因芯片法的灵敏度、特异度和符合率。结果 182例疑似结核性脓胸患者中,135例临床确诊为结核性脓胸,基因芯片法和快速培养法的特异性均为95.7%(45/47),灵敏度分别为48.9%(66/135)和26.7%(36/135),二者比较有统计学差异(χ2=80.5,P<0.05)。基因芯片法检测RFP耐药性灵敏度、特异度和符合率均为100%;而检测INH耐药性的灵敏度、特异度和符合率分别为50.0%(1/2)和97.1%(33/34)和94.4%。结论基因芯片技术检测分枝杆菌具有较高的、特异性,可快速鉴别出非结核分枝杆菌,且可有效检测结核分枝杆菌异烟肼和利福平的耐药性,对结核性脓胸的早期诊断与治疗有重要意义。服务

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收稿日期: 2016-12-09
PACS: R378.9

通讯作者: 陈晓红,Email: cxhong6886@126.com
引用本文:
林秀华, 赖国祥, 陈雨燕, 郑丹, 周银发, 陈晓红. 基因芯片技术检测分枝杆菌和异烟肼、利福平耐药性在结核性脓胸诊断中的应用[J]. 中国人兽共患病学报, 2017, 33(8): 720-723. LIN Xiu-hua, LAI Guo-xiang, CHEN Yu-yan, ZHENG Dan, ZHOU Yin-fa, CHEN Xiao-hong. Gene chip technology for detecting mycobacteria and Mycobacterium tuberculosis resistance of isoniazid and rifampin in the application of tuberculous empyema. Chinese Journal of Zoonoses, 2017, 33(8): 720-723.

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