您养的细胞还好吗？我医用离心机们一起来探索细胞之旅吧

作者：王韵壹、李英、熊慧卿

活动目的：

20世纪80年代重组哺乳动物细胞系建立以来，并且随着分子生物学技术的不断发展，细胞用于生物制品特别是疫苗的生产、检测等方面越来越多。大肠杆菌、酵母、昆虫细胞和哺乳动物细胞都可以用于蛋白的表达，但真核细胞较于原核表达系统，具有高质量的生物活性蛋白质，同时在操作及应用中可分为瞬时转染短暂表达和稳定转染持续表达。因此，在疫苗生产过程前，科研人员应结合病毒或细菌生物学及免疫学特性，克服真核细胞表达产量低、操作系统复杂等缺点，选择合适的细胞系、培养基、培养条件和培养技术，以确保疫苗或其他生物制品在安全性和有效性的同时，还能保证经济收益。现在适用于规模化培养的细胞包扩悬浮细胞、贴壁细胞核半贴壁细胞培养。有生物反应器、细胞工厂和微载体等等细胞培养技术。除了生产，与细胞相关制品的质量控制与保证，也是所有生物制品生产人员更关注的问题，这对于工艺设计、种子库的来源和建立、工艺流程的确认和验证、产品检定方法的统一、人员培训都有更加严格的要求。

因此，我们特搜集了关于疫苗及其他生物制品在细胞培养方面的文献共33篇文献，诚邀从事细胞培养或对其有兴趣的您，贡献您少量宝贵的业余时间进行翻译，便于同行资料信息的互通与交流。最终，我们会将译文和原文全文分享，在此诚挚欢迎各同仁踊跃报名参与此次翻译活动。

参与对象：

从事细胞培养以及对细胞培养感兴趣的同仁

参与流程：

1.猛戳“阅读原文”，填报个人信息进行预报名；

2.加微信号cbp510(可扫二维码加入)，了解参与活动的相关规则；

3.通过翻译测试稿进行筛选，根据翻译的结果遴选参与人员；

4.拆分章节/段落，统一分发相关翻译文件；

5.安排参与人员对翻译作品进行相互校对；

6.完成后的译本仅参与者共享；

7.对积极参与并按时完成的同仁，分享中文译文全文。

文献列表

1. A Consensus Genome-scale Reconstruction of chinese hamster ovary cell metabolism

展开全文

2. A microneedle patch containing Mealse vaccine is immunogenic in non-human primate

3. A Novel Seed-Train Process using high-density cell banking a disposable bioreactor and perfusion technologies

4. Advanced Cell Culture Techniques for Cancer Drug Discovery

5. Advancement in bioprocess technology_ parallels between microbial natural products and cell culture biologics

6. Applicability of predictive toxicology methods for monoclonal antibody therapeutics status quo and scope

7. Assessment of packed bed bioreactor systems in the production of viral vaccine

8. Bioreactor concepts for cell culture-based viral vaccine production

9. Cell Culture for Production of Insecticidal Viruses

10. Cell culture processes for monoclonal antibody production

11. Cell substrates for the production of viral vaccines

12. CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion

13. Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the wave bioreactor

14. Current and Emerging Cell Culture Manufacturing technology for influenza vaccine

15. Designing cell lines for viral vaccine production_where do we stand

16. Engineering Cells to Improve Protein Expression

17. intensified Design of Experiments for upstream bioreactors

18. Lifecycle Management for Recombinant protein production using mammalian cell culture technology

19. Matrix and Backstage_ Cellular Substrates for Viral Vaccines

20. Micro-scaffold Array Chip for upgrading cell-based High-throughput drug testing to 3D using benchtop equipment

21. Model-based design of experiments for cellular process

22. Optimum Infection Conditions for Recombinant Protein Production in insect cell suspension culture

23. Overexpression of membrane proteins in mammalian cells for structural studies

24. Overexpression of YY1 increases the protein production in mammalian cells

25. Quality by controlTowards model predictive control of mammalian cell culturebioprocesses

26. Recombinant protein production from stable mammalian cell lines and pools

27. Scaled-up Manufacturing of Recombinant antibodies produces by plant cells in a 200L orbitally-shaken disposable bioreactor

28. Stable mammalian producer cell lines for structural biology

29. Three-Dimensional Cell Cultures in Drug discovery and development

30. Vaccines for the 21st century

31. vero cell platform in vaccine production moving towards cell culture-based viral vaccine

32. Viruses as vaccine vectors for infectious dieases and cancer