对流免疫电泳缓冲液及电泳介质的筛选
文章导航 > 实验科学与技术 > > 19(1): 115-118.
周瑞雪, 李冬菊, 隋鲜鲜. 对流免疫电泳缓冲液及电泳介质的筛选[J]. 实验科学与技术, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
引用本文: 周瑞雪, 李冬菊, 隋鲜鲜. 对流免疫电泳缓冲液及电泳介质的筛选[J]. 实验科学与技术, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
ZHOU Ruixue, LI Dongju, SUI Xianxian. Selection of Buffer Solution and Medium for Counter-immunoelectrophoresis[J]. Experiment Science and Technology, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
Citation: ZHOU Ruixue, LI Dongju, SUI Xianxian. Selection of Buffer Solution and Medium for Counter-immunoelectrophoresis[J]. Experiment Science and Technology, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
周瑞雪, 李冬菊, 隋鲜鲜. 对流免疫电泳缓冲液及电泳介质的筛选[J]. 实验科学与技术, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
引用本文: 周瑞雪, 李冬菊, 隋鲜鲜. 对流免疫电泳缓冲液及电泳介质的筛选[J]. 实验科学与技术, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
ZHOU Ruixue, LI Dongju, SUI Xianxian. Selection of Buffer Solution and Medium for Counter-immunoelectrophoresis[J]. Experiment Science and Technology, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
Citation: ZHOU Ruixue, LI Dongju, SUI Xianxian. Selection of Buffer Solution and Medium for Counter-immunoelectrophoresis[J]. Experiment Science and Technology, 2021, 19(1): 115-118. doi: 10.12179/1672-4550.20190444
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对流免疫电泳缓冲液及电泳介质的筛选 doi: 10.12179/1672-4550.20190444
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基金项目: 复旦大学国家级大学生创新创业项目（18052）
详细信息出版历程
收稿日期: 2019-11-25
修回日期: 2020-11-16
网络出版日期: 2020-12-28
刊出日期: 2021-02-27
Selection of Buffer Solution and Medium for Counter-immunoelectrophoresis
Abstract: In order to select a safe, economical and effective buffer solution and medium for counter-immunoelectrophoresis (CIEP), six buffer solutions with pH value of 8.6 were selected from common buffer solutions for electrophoresis, with agar and agarose used respectively as the electrophoresis media, to observe the formation of white precipitation line of antigens and antibodies after electrophoresis. The results showed that Tris-HCl buffer was the best of the six buffer solutions. Both agar and agarose gels produced significant white precipitation lines, and the white precipitators became shallower with the decrease of antigen concentration. The effects of agarose used as the electrophoresis media in barbiturate and barbiturate sodium, TAE, Gly-NaOH and NaCl-NaOH buffers were better than those of agar. The TGS buffer was the worst. It was concluded that Tris-HCl, Barbitone and barbitone sodium, TAE, Gly-NaOH and NaCl-NaOH buffer could all be used as buffer solution for counter immunoelectrophoresis. However, Tris-HCl was the best, followed by TAE and Gly-NaOH, in terms of safety, economy and experimental effect. Therefore, Tris-HCl will be used as buffer solution for counter-immunoelectrophoresis in the future immunological experiments.
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图 1 抗原抗体加样模式图
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表 1 6种对流免疫电泳缓冲液配方
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