catenin信号通路影响哮喘小鼠气道重塑机制的研究

作者：王韵壹、李英、熊慧卿

摘要： 目的探讨白三烯受体拮抗剂影响Wnt7b、β-catenin及c-Myc表达，干预哮喘小鼠气道重塑机制的研究。方法采用卵蛋白（OVA）致敏和激发建立小鼠哮喘模型。将小鼠分为对照组、哮喘组以及孟鲁司特钠组。HE染色观察各组小鼠气道炎症情况；ELISA法检测血清OVA-sIgE水平；实时PCR和Western blotting分别定量分析肺组织中Wnt7b、β-catenin及c-Myc mRNA和蛋白表达水平；运用图像采集系统测定支气管管腔周长（PBM）、支气管管壁面积（WAt）、支气管管壁平滑肌面积（WAm）和平滑肌细胞计数（N），上述指标用PBM标准化。结果哮喘组小鼠血清OVA-sIgE表达水平明显升高（P < 0.05）。Western blotting以及实时PCR结果显示哮喘组Wnt7b、β-catenin及c-Myc蛋白和mRNA较对照组和孟鲁司特钠组表达均显著升高（P < 0.05）。气道重塑指标WAt/PBM、WAm/PBM孟鲁司特钠组均低于哮喘组（P < 0.05）。结论 Wnt/β-catenin信号通路可能是哮喘发病机制中的重要因素，白三烯受体拮抗剂（孟鲁司特钠）可能通过影响Wnt7b、β-catenin及c-Myc的表达干预哮喘气道重塑。

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R562.25

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蒋昱, 黄传君, 李泽, 张才擎. 白三烯受体拮抗剂通过Wnt/β-catenin信号通路影响哮喘小鼠气道重塑机制的研究[J]. 中国医科大学学报, 2018, 47(3): 226-230.

JIANG Yu, HUANG Chuanjun, LI Ze, ZhANG Caiqing. Effects of a Leukotriene Receptor Antagonist on Airway Remodeling in Asthmatic Mice via the Wnt/β-catenin Signaling Pathway[J]. Journal of China Medical University, 2018, 47(3): 226-230.

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